h01-volume

Reconstructions and analyses from the Lichtman laboratory at Harvard University and Connectomics group at Google H01 human cortex serial EM volume.

Attribution and data: https://h01-release.storage.googleapis.com/data.html
Citation: https://h01-release.storage.googleapis.com/paper.html

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View notebook folder for analysis and visualization tools

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Fission between two mitochondria

Two mitochondria appear to be in the end stages of fission. Viewing the z-plane above and below the current plane of focus shows that there is a thin bit of membrane connecting the two mitochondria together. Mitochondria are dynamic, moving along the cytoskeleton, fusing together and breaking apart. This dynamic transition is frozen in time here in this fixed electron micrograph.

View in Neuroglancer

fission between two mitochondria in a pyramidal dendrite

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Astrocyte rendered using color and opacity gradient

rendering of an astrocyte from the H01 volume using a color and opacity gradient

Astrocytes have massive cell surface area, making it hard to visualize in a 3D reconstruction (it appears to be a dense cloud). In the notebook folder, there is a vtk visualization notebook that uses a color and opacity gradient that makes the astrocyte leaflets more readily apparent.